Biosynthesization of ZnO/CuO Nanocomposites in Orange Peel Crude Extract for Antibacterial Activities
Abstract
Nanoparticles (NPs) in particular ZnO and CuO are inorganic nanomaterials used in application areas such as electronics, communication, chemical/biological sensors, cosmetics, environmental remediation, biomedicinal industry, energy preservation, photocatalysis, and microbial growth inhibition. This study focused on the biosynthesis of ZnO NPs, CuO NPs, and ZnO/CuO nanocomposites (NCs) in orange fruit extract to investigate the growth inhibition of gram-positive and gram-negative bacteria. The crystal structure, functional group, and energy band gap of as-synthesized NPs and NCs were characterized with the aid of X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), and Ultra Violet-Visible (UV-Vis) spectroscopic techniques, respectively. Consequently, the XRD results confirmed the formation of a hexagonal wurtzite phase, and the average crystallite sizes of the nanomaterials of 22.71-33.89, 16.46-33.04 and 16.76-26.89 nm for ZnO NPs, CuO NPs, and ZnO/CuO NCs synthesized with and without orange peel extract, respectively while the vibrational stretching obtained around 476 and 564 cm-1 confirmed the presence of Zn-O, and Cu-O bond, respectively. The characteristic absorption spectrum observed at 285 nm supported the synthesization of ZnO/CuO NCs within a very narrow energy bandgap. Furthermore, the antibacterial activity of the ZnO/CuO with orange peel extract (WE) NCs, ZnO (WE) and CuO (WE) were significantly higher as compared to that without orange fruit extracts (WoE) NPs/NCs. The results show that the NPs and NCs synthesized WE had a high potential growth inhibition zone against gram-positive bacteria (S. aureus) ranges from 16.67 to 12.00 mm and gram-negative bacteria (P. aeroginosa) ranges 16.00 to 12.30 mm. In this study, we have discovered that the green synthesized ZnO/CuO NCs can be introduced as a promising anti-bacterial agent and so applicable to cure microbial strain-based infectious diseases.
Similar Articles
- Asefa Keneni, Geda Kebede, Growth, Yield, and Biological Efficiency of Oyster Mushroom (Pleurotus ostreatus) Grown on Different Substrate Mix Ratios , Journal of Science and Sustainable Development: Vol. 4 No. 2 (2016)
- Berhanu Alemu, Macroeconomic Determinates of Private Fixed Investment in Large and Medium Scale Manufacturing Industries of Ethiopia: A Time Series Analysis , Journal of Science and Sustainable Development: Vol. 2 No. 2 (2014)
- Hiwot Teshome, Kibru Teshome, Adamneh Dagne, Potentials and Challenges of Smallholder Fish Farming in Ethiopia: The Case of South West and West Showa Zones, Oromiya, Ethiopia , Journal of Science and Sustainable Development: Vol. 4 No. 1 (2016)
- Hadush Gebretsadik , Firew Mekbib, Eyasu Abraha, Influence of Cytokinin and Auxin Types and Concentrations on In Vitro Shoot and Root Regeneration of Cactus Pear [(Opuntia ficus-indica (L.) Mill.] , Journal of Science and Sustainable Development: Vol. 1 No. 1 (2013)
- Abate Sisay Solomon, In vitro Plantlet Regeneration from Nodal Explants of Anchote [Coccinia abyssinica (Lam.) Cong.] Genotypes , Journal of Science and Sustainable Development: Vol. 8 No. 1 (2020)
- Fikiru Temesgen, Lachisa Amina, Determinants of Households’ Decision to Save at Household Level: Evidence from Small holder Farmers’ of Ambo District, Oromia National Regional State, Ethiopia , Journal of Science and Sustainable Development: Vol. 9 No. 1 (2021)
- Girma Dawit, Tamado Tana, Jemal Abdulahi, Effects of Seed and NPS Fertilizer Rates on Yield Components and Yield of Bread Wheat (Triticum aestivum L.) in East Badawacho District, Southern Ethiopia , Journal of Science and Sustainable Development: Vol. 9 No. 2 (2021)
- Tsegaye Dabessa, Alemu Lencho, Thangavel Selvaraj, Jemal Tola, Gezahegne Getaneh, Distribution and Pathogen Characterization of Chickpea Fusarium Wilt (Fusarium oxysporum f.sp. ciceri) in the West and Southwest Shewa Zones of Oromia Regional State, Ethiopia , Journal of Science and Sustainable Development: Vol. 14 No. 2 (2026)
- Abdisa Gesesse, Tesfaye Balemi, P. Natarajan, Yosef Amha, Effect of Vetiver Grass Headges in Maintaining Soil Fertility and Productivity at Anno Agro Industry Farm, Gobu Sayo District, Oromiya Region, Ethiopia , Journal of Science and Sustainable Development: Vol. 1 No. 1 (2013)
- Haji Kedir, Yohannes Gebremichael, Solomon Tekalign, Assessment of Water Supply Systems in Karrayu Pastoral Area, Oromiya Region, Ethiopia , Journal of Science and Sustainable Development: Vol. 3 No. 1 (2015)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Busha Jima, Misganu Yonas, Tibebu Alemu, Jabane Dinkisa, Bulti Abdisa Kerayu, Phytochemical Characterization and Invitro Evaluation of Antibacterial and Antioxidant Activities of Dodonaea angustifolia Leaves in Ambo District, Ethiopia , Journal of Science and Sustainable Development: Vol. 13 No. 1 (2025)
- Tibebu Alemu, Teshome Abebe, Ahmed Abdurauf, Girmaye Asefa, Bulti Abdisa, Gemechis Fikadu, Kumela Dabesa, Bekele Negasa, Synergetic Effect of Ni-CuO Nanocomposites with Activated Carbon Synthesized from Zeamays Bark for the Removal of Methylene Blue Dye , Journal of Science and Sustainable Development: Vol. 13 No. 2 (2025)
- Tibebu Alemu, Taye Bekele, Girmaye Asefa, Bulti Abdisa Kerayu, Surface Kinetic and Thermodynamic Behaviors of Cu-NiO/PANI Assisted Photodegradation of Tris(4-(Dimethylamino) Phenyl) Methylium Chloride (TDPM) , Journal of Science and Sustainable Development: Vol. 11 No. 2 (2023)